Development and validation of a rapid immunochromatographic assay for detection of Middle East respiratory syndrome coronavirus antigen in dromedary camels.
Identifieur interne : 001828 ( Main/Exploration ); précédent : 001827; suivant : 001829Development and validation of a rapid immunochromatographic assay for detection of Middle East respiratory syndrome coronavirus antigen in dromedary camels.
Auteurs : Daesub Song [Corée du Sud] ; Gunwoo Ha [Corée du Sud] ; Wissam Serhan [Émirats arabes unis] ; Yassir Eltahir [Émirats arabes unis] ; Mohammed Yusof [Émirats arabes unis] ; Farouq Hashem [Émirats arabes unis] ; Elsaeid Elsayed [Émirats arabes unis] ; Bahaaeldin Marzoug [Émirats arabes unis] ; Assem Abdelazim [Émirats arabes unis] ; Salama Al Muhairi [Émirats arabes unis]Source :
- Journal of clinical microbiology [ 1098-660X ] ; 2015.
Descripteurs français
- KwdFr :
- Animaux, Antigènes viraux (analyse), Chameaux (virologie), Chromatographie d'affinité (), Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification), Fosse nasale (virologie), Infections à coronavirus (diagnostic), Infections à coronavirus (médecine vétérinaire), Infections à coronavirus (virologie), Reproductibilité des résultats, Sensibilité et spécificité, Virologie.
- MESH :
- analyse : Antigènes viraux.
- diagnostic : Infections à coronavirus.
- isolement et purification : Coronavirus du syndrome respiratoire du Moyen-Orient.
- médecine vétérinaire : Infections à coronavirus.
- virologie : Chameaux, Fosse nasale, Infections à coronavirus.
- Animaux, Chromatographie d'affinité, Reproductibilité des résultats, Sensibilité et spécificité, Virologie.
English descriptors
- KwdEn :
- Animals, Antigens, Viral (analysis), Camelus (virology), Chromatography, Affinity (methods), Coronavirus Infections (diagnosis), Coronavirus Infections (veterinary), Coronavirus Infections (virology), Middle East Respiratory Syndrome Coronavirus (isolation & purification), Nasal Cavity (virology), Reproducibility of Results, Sensitivity and Specificity, Virology.
- MESH :
- chemical , analysis : Antigens, Viral.
- diagnosis : Coronavirus Infections.
- isolation & purification : Middle East Respiratory Syndrome Coronavirus.
- methods : Chromatography, Affinity.
- veterinary : Coronavirus Infections.
- virology : Camelus, Coronavirus Infections, Nasal Cavity.
- Animals, Reproducibility of Results, Sensitivity and Specificity, Virology.
Abstract
We present here a rapid immunochromatographic assay for the detection of Middle East respiratory syndrome coronavirus (MERS-CoV) antigen in the nasal swabs of dromedary camels. The assay is based on the detection of MERS-CoV nucleocapsid protein in a short time frame using highly selective monoclonal antibodies at room temperature. The relative sensitivity and specificity of the assay were found to be 93.90% and 100%, respectively, compared to that of the UpE and open reading frame 1A (Orf1A) real-time reverse transcriptase PCR (RT-PCR). The results suggest that the assay developed here is a useful tool for the rapid diagnosis and epidemiological surveillance of MERS-CoV infection in dromedary camels.
DOI: 10.1128/JCM.03096-14
PubMed: 25631809
Affiliations:
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Dhabi</wicri:regionArea><wicri:noRegion>Abu Dhabi</wicri:noRegion></affiliation></author><author><name sortKey="Hashem, Farouq" sort="Hashem, Farouq" uniqKey="Hashem F" first="Farouq" last="Hashem">Farouq Hashem</name><affiliation wicri:level="1"><nlm:affiliation>Veterinary Laboratories Division, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi, United Arab Emirates.</nlm:affiliation><country xml:lang="fr">Émirats arabes unis</country><wicri:regionArea>Veterinary Laboratories Division, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi</wicri:regionArea><wicri:noRegion>Abu Dhabi</wicri:noRegion></affiliation></author><author><name sortKey="Elsayed, Elsaeid" sort="Elsayed, Elsaeid" uniqKey="Elsayed E" first="Elsaeid" last="Elsayed">Elsaeid Elsayed</name><affiliation wicri:level="1"><nlm:affiliation>Veterinary Services Section, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi, United Arab Emirates.</nlm:affiliation><country 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wicri:level="1"><nlm:affiliation>Veterinary Services Section, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi, United Arab Emirates.</nlm:affiliation><country xml:lang="fr">Émirats arabes unis</country><wicri:regionArea>Veterinary Services Section, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi</wicri:regionArea><wicri:noRegion>Abu Dhabi</wicri:noRegion></affiliation></author><author><name sortKey="Al Muhairi, Salama" sort="Al Muhairi, Salama" uniqKey="Al Muhairi S" first="Salama" last="Al Muhairi">Salama Al Muhairi</name><affiliation wicri:level="1"><nlm:affiliation>Veterinary Laboratories Division, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi, United Arab Emirates salama.almuhairi@adfca.ae.</nlm:affiliation><country wicri:rule="url">Émirats arabes unis</country><wicri:regionArea>Veterinary Laboratories Division, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi</wicri:regionArea><wicri:noRegion>Abu 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d'affinité ()</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification)</term><term>Fosse nasale (virologie)</term><term>Infections à coronavirus (diagnostic)</term><term>Infections à coronavirus (médecine vétérinaire)</term><term>Infections à coronavirus (virologie)</term><term>Reproductibilité des résultats</term><term>Sensibilité et spécificité</term><term>Virologie</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antigens, Viral</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Antigènes viraux</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Chromatography, Affinity</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Chameaux</term><term>Fosse nasale</term><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Camelus</term><term>Coronavirus Infections</term><term>Nasal Cavity</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Reproducibility of Results</term><term>Sensitivity and Specificity</term><term>Virology</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Chromatographie d'affinité</term><term>Reproductibilité des résultats</term><term>Sensibilité et spécificité</term><term>Virologie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We present here a rapid immunochromatographic assay for the detection of Middle East respiratory syndrome coronavirus (MERS-CoV) antigen in the nasal swabs of dromedary camels. The assay is based on the detection of MERS-CoV nucleocapsid protein in a short time frame using highly selective monoclonal antibodies at room temperature. The relative sensitivity and specificity of the assay were found to be 93.90% and 100%, respectively, compared to that of the UpE and open reading frame 1A (Orf1A) real-time reverse transcriptase PCR (RT-PCR). The results suggest that the assay developed here is a useful tool for the rapid diagnosis and epidemiological surveillance of MERS-CoV infection in dromedary camels. </div></front></TEI><affiliations><list><country><li>Corée du Sud</li><li>Émirats arabes unis</li></country></list><tree><country name="Corée du Sud"><noRegion><name sortKey="Song, Daesub" sort="Song, Daesub" uniqKey="Song D" first="Daesub" last="Song">Daesub Song</name></noRegion><name sortKey="Ha, Gunwoo" sort="Ha, Gunwoo" uniqKey="Ha G" first="Gunwoo" last="Ha">Gunwoo Ha</name></country><country name="Émirats arabes unis"><noRegion><name sortKey="Serhan, Wissam" sort="Serhan, Wissam" uniqKey="Serhan W" first="Wissam" last="Serhan">Wissam Serhan</name></noRegion><name sortKey="Abdelazim, Assem" sort="Abdelazim, Assem" uniqKey="Abdelazim A" first="Assem" last="Abdelazim">Assem Abdelazim</name><name sortKey="Al Muhairi, Salama" sort="Al Muhairi, Salama" uniqKey="Al Muhairi S" first="Salama" last="Al Muhairi">Salama Al Muhairi</name><name sortKey="Elsayed, Elsaeid" sort="Elsayed, Elsaeid" uniqKey="Elsayed E" first="Elsaeid" last="Elsayed">Elsaeid Elsayed</name><name sortKey="Eltahir, Yassir" sort="Eltahir, Yassir" uniqKey="Eltahir Y" first="Yassir" last="Eltahir">Yassir Eltahir</name><name sortKey="Hashem, Farouq" sort="Hashem, Farouq" uniqKey="Hashem F" first="Farouq" last="Hashem">Farouq Hashem</name><name sortKey="Marzoug, Bahaaeldin" sort="Marzoug, Bahaaeldin" uniqKey="Marzoug B" first="Bahaaeldin" last="Marzoug">Bahaaeldin Marzoug</name><name sortKey="Yusof, Mohammed" sort="Yusof, Mohammed" uniqKey="Yusof M" first="Mohammed" last="Yusof">Mohammed Yusof</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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